DOI : https://doi.org/10.29165/ajarcde.v10i2.1014

Synthesis of Hydroxyapatite from Snail Shells Using the Precipitation Method

rahel aditya amara putri (1), moh rifqi maulana (2), erwan adi saputro (3), caecillia pujiastuti (4), Ni ketut sari (5)
(1) Department of Chemical Engineering, Universitas Pembangunan Nasional “Veteran” Jawa Timur, Surabaya 60294, Indonesia.
(2) Department of Chemical Engineering, Universitas Pembangunan Nasional “Veteran” Jawa Timur, Surabaya 60294, Indonesia.
(3) Department of Chemical Engineering, Universitas Pembangunan Nasional “Veteran” Jawa Timur, Surabaya 60294, Indonesia.
(4) Department of Chemical Engineering, Universitas Pembangunan Nasional “Veteran” Jawa Timur, Surabaya 60294, Indonesia.
(5) Department of Chemical Engineering, Universitas Pembangunan Nasional “Veteran” Jawa Timur, Surabaya 60294, Indonesia.
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rahel aditya amara putri, moh rifqi maulana, erwan adi saputro, caecillia pujiastuti, & Ni ketut sari. (2026). Synthesis of Hydroxyapatite from Snail Shells Using the Precipitation Method. AJARCDE (Asian Journal of Applied Research for Community Development and Empowerment), 10(2), 211–217. https://doi.org/10.29165/ajarcde.v10i2.1014
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Hydroxyapatite (HA) is a bioceramic widely used in biomedical applications due to its excellent biocompatibility and bioactivity. This study aims to synthesise hydroxyapatite from rice field snail shells (Pila ampullacea) using the precipitation method and to evaluate the effects of phosphoric acid (H?PO?) concentration and heating time on the synthesised hydroxyapatite's characteristics. The synthesis was conducted by varying H?PO? concentration from 0.25 to 2 M and heating time from 1 to 3 hours at a calcination temperature of 800°C. The resulting hydroxyapatite was characterized by SEM-EDX, XRF, XRD, and FTIR analyses, and the Ca/P ratio was optimized using Response Surface Methodology (RSM). SEM-EDX analysis revealed porous and agglomerated surface morphology, indicating successful thermal decomposition of calcium carbonate into calcium oxide                precursor. XRF results showed that increasing H?PO? concentration and heating time significantly reduced the Ca/P ratio toward the stoichiometric value of                hydroxyapatite (1.67), with optimum conditions achieved at 1.5–2 M H?PO? and         2–2.5 hours heating time. XRD analysis confirmed the formation of crystalline              hydroxyapatite with an average crystal size of 14.62 nm and crystallinity degree of 81.23%. FTIR spectra identified characteristic phosphate functional groups,           confirming hydroxyapatite formation. The RSM optimization demonstrated that both H?PO? concentration and heating time significantly influence the Ca/P ratio. These findings indicate that rice field snail shells have strong potential as a                 sustainable and cost-effective alternative calcium source for hydroxyapatite synthesis in biomaterial applications.


Contribution to Sustainable Development Goals (SDGs):
SDG 3: Good Health and Well-Being
SDG 9: Industry, Innovation, and Infrastructure
SDG 12: Responsible Consumption and Production

[1] F. Behmagam, S. M. Dhiaa & A. H. A. Hussein, ‘Hydroxy-apatite–Polymer Nanocomposites for Drug Delivery Appli-cations: A Mini Review’, European Journal of Medicinal Chemistry Reports, 1(1), 1-13, 2024.

[2] A. Iatsenko, O. Syech, A. Nikolenko & S. Stelmakh, 'Struc-ture Investigation Of Highly Porous Bioceramics Based On Biogenic Hydroxyapatite With Graphene Oxide Coating’, Journal Results In Surfaces And Interfaces, 2(1), 19-38, 2024.

[3] M. S. F. Hussin, H. Z. Abdullah, M. I. Idris & M. A. A. Wahap,’Extraction Of Natural Hydroxyapatite For Biomedi-cal Applications A Review’, Journal Heliyon, 8(1), 10-19, 2022.

[4] S. Patil, S. Thanikodi, S. Palaniyappan, J Giri, A. O Hourani & M. Azizi,’ Sustainable synthesis of marine-derived hy-droxyapatite for biomedical applications: a systematic review on extraction methods and bioactivity’, Advanced Manufac-turing:Polymer & Composite Science, 11(1), 1-30, 2025.

[5] H. Y. Ahmed, N. Safwat, R. Shehata, & E. H. Althubaiti,’ Synthesis of Natural Nano-Hydroxyapatite from Snail Shells and Its Biological Activity: Antimicrobial, Antibiofilm, and Biocompatibility’, Journal Membraness, 12(4), 1-16, 2022.

[6] Z. Kareem & E. Eyiler,’Synthesis Of Hydroxyapatite From Eggshells Via Wet Chemical Precipitation: A Review’, RSC Advances, 14(1), 21439–21452, 2024.

[7] W. Chaiyacet, R. Junggoth, T. Donprajum, S. Yasaka, R. Chaiyacet & E. Kanchanatip, ’Microwave assisted hydro-thermal synthesis of hydroxyapatite from fermented fish by product for removal of lead from contaminated water’, Beni-Suef University Journal of Basic and Applied Scienc-es,14(116), 1-22, 2025.

[8] H. Atta, K. R. Mahmoud, E. I. Salim, E. Elmohsnawy & A. El-Shaer, ‘Correlation Between Positron Annihilation Lifetime and Photoluminescence Measurements for Calcined Hydroxyapatite’, Scientific Reports, 14(1), 10370, 2024.

[9] N. Keanjun, T. Rattanawongwiboon, P. Sricharoen & S. Laksee, ‘Ultrasound-Assisted Formation of Composite Materials from Fish Scale Waste Hydroxyapatite in the Pres-ence of Gamma-Irradiated Chitosan for The Removal of Malachite Green’, RSC Advances, 14(1), 29737–29747, 2024.

[10] S. Balhuc, R. Campian, A. Labunet, M. Negucioiu S. Budu-ru & A. Kui, ’Dental Applications of Systems Based on Hy-droxyapatite Nanoparticles an Evidence-Based Update’, Journal Crystals, 11(1), 674, 2021.

[11] M. U. Munir, S. Salman, A. Ihsan & T. Elsaman,’ Synthe-sis, Characterization, Functionalization and Bio-Applications of Hydroxyapatite Nanomaterials: An Overview’, Interna-tional Journal of Nanomedicine, 17(1),1903-1925, 2022.

[12] N. Tian, G. Zhang, P. Yan, P. Li, Z. Feng & X. Wang, ‘Simulation and Experimental Study on the Effect of Super-heat on Solidification Microstructure Evolution of Billet in Continuous Casting’, Materials, 17(3), 1-14, 2024.

[13] W. Khoo, F. M. Nor, H. Ardhyananta & D. Kurniawan, ‘Preparation of Natural Hydroxyapatite from Bovine Bone Based on Calcination at Different Temperatures’, Procedia Manufacturing, 2, 98-102. 2015.

[14] F. Gao, W. Liang, Q. Chen, B Chen & Y. Liu, ‘A Curcu-min-Decorated Nanozyme with ROS Scavenging and Anti-Inflammatory Properties for Neuroprotec-tion’,Nanomaterials, 14(5), 1-15. 2024.

[15] S. V. Dorozhkin,’Calcium orthophosphates (CaPO?) Occur-rence and properties’, Progress in Biomaterials,5(1–2), 9–70, 2016.

[16] Y. Zou, Y. Wang & J. Wang, ‘Preparation of hydroxyapatite and its elimination of excess fluoride from aqueous solu-tion’, RSC Advances, 14(1), 26103-26114, 2024.

[17] J. Venkatesan, R. V. Anchan, S. S. Murugan, S. Anil & S. K. Kim, ‘Natural Hydroxyapatite Based Nanobiocomposites and Their Biomaterials To Cell Interaction for Bone Tissue Engineering’, Discover Nano, 19(1), 169, 2024.

[18] S. L. Bee, S. N. F. M. Noor, A. U Hamid & Z. A. A. Ha-mid, 'Effect of calcination temperature on the physicochemi-cal properties of natural hydroxyapatite derived from Catla fish bone', Journal of Physics: Conference Series, 2907(1), 1-7, 2024.

[19] S. C. Wu, H. C. Hsu, W. H. Wu & W. F. Ho, ‘Enhancing Bioactivity and Mechanical Properties of Nano-Hydroxyapatite Derived from Oyster Shells through Hydro-thermal Synthesis’, Nanomaterials, 14(15), 1-16, 2024.

[20] E. A. A. Aal, D. E. Sayed & H. M. A. Ghafar, ‘Hydrother-mally synthesized nanoscale carbonated hydroxyapatite with calcium carbonates derived from green mussel shell wastes’, Discover Applied Sciences, 6(143), 2024.

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