Calcium Chloride Treatment Patterns During Germination Influence Calcium Accumulation and Physicochemical Characteristic of of Red Rice
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Germination in red rice aims to improve its quality, particularly its texture. However, this germination process produces relatively long sprouts that can interfere with the quality of red rice when consumed. Therefore, calcium chloride is utilized with different application patterns to suppress sprout elongation while simultaneously enhancing calcium accumulation. Red rice (Inpari 24 variety) was germinated using a partially circulated germinator with variations in calcium chloride treatment patterns and germination duration. The physicochemical characteristics of ungerminated and germinated red rice exhibited differences, specifically in germination quality, calcium, color, pasting profile, and total sugar content. Germination without calcium chloride treatment and germination with full calcium chloride treatment at the initial stage significantly affected the germination rate, sprout length, calcium, and pasting profile of the red rice. Germination under each distinct calcium chloride application pattern successfully suppressed red rice sprout elongation. The results indicated that full calcium chloride treatment at the initial stage produced the lowest germination rate and sprout length (74.67–88.00% and 0.66–1.24 mm, respectively). Germination with calcium chloride treatment at the intermediate-stage and in a gradual manner reduced the sprout length of the red rice. The treatment with calcium chloride also increased calcium levels, an essential mineral for humans. Full treatment of calcium chloride at the initial stage yielded germinated red rice with the highest calcium (228.06–252.20 mg/100g). The different calcium chloride treatment patterns did not significantly affect the color, pasting profile, and total sugar of the germinated red rice; however, an increase was observed from the ungerminated red rice to the germinated red rice.
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[1] A. Singh, B. Sharma, and B. Singh, “Germination Behaviour , Physico-Nutritional Properties , And Diastase Activity Of Brown Rice Influenced By Germination Time And Temperature,” Acta Aliment., vol. 47, no. 1, pp. 70–79, 2018, doi: 10.1556/066.2018.47.1.9.
[2] D. Fitriyah, D. P. Ayu, S. D. Puspita, R. C. Kartika, and M. Ubaidillah, “Kandungan Nutrisi dan Aktivitas Antimikroba Ekstrak Beras Merah Nutrient Content and Antimicrobial Activity of Red Rice Extract,” J. Pangan dan Gizi, vol. 12, no. 2, pp. 30–36, 2022.
[3] S. B. Patil and M. K. Khan, “Germinated Brown Rice as A Value Added Rice Product: A Review,” J. Food Sci. Technol., vol. 48, no. 6, pp. 661–667, 2011, doi: 10.1007/s13197-011-0232-4.
[4] A. C. Oliveira, C. Pegoraro, and V. E. Viana, The Future of Rice Demand: Quality Beyond Productivity, no. March. 2020. doi: 10.1007/978-3-030-37510-2.
[5] D. H. Cho and S. T. Lim, “Germinated Brown Rice and Its Bio-functional Compounds,” Food Chem., vol. 196, pp. 259–271, 2016, doi: 10.1016/j.foodchem.2015.09.025.
[6] H. Munarko, A. B. Sitanggang, F. Kusnandar, and S. Budijanto, “Kecambah Beras Pecah Kulit?: Proses Produksi dan Karakteristiknya Germinated Brown Rice: Production Process and Its Characteristics A R T I K E L,” J. Pangan, vol. 28, no. 3, pp. 1–14, 2019.
[7] V. Yemmireddy, A. Adhikari, and J. Moreira, “Efect of Ultraviolet Light Treatment on Microbiological Safety and Quality of Fresh Produce?: An overview,” Front. Nutr., vol. 9, no. 4, 2022.
[8] S. Tosheva, G. Desheva, E. Valchinova, and A. Pencheva, “Effect of Calcium Chloride Concentration on Seed Germination and Young Seedling Parameters in Rice ( Oryza Sativa L.),” Bulg. One Heal., vol. 1, no. 3, pp. 30–39, 2024.
[9] J. Wu et al., “Calcium Dynamics During the Growth of Agaricus bisporus: Implications for Mushroom Development and Nutrition,” Chem. Biol. Technol. Agric., vol. 10:99, pp. 1–18, 2023, doi: 10.1186/s40538-023-00471-y.
[10] E. Borghesi et al., “Effects of Calcium and Salinity Stress on Quality of Lettuce in Soilles Culture,” J. Plant Nutr., vol. 36, no. 5, pp. 677–690, 2013, doi: 10.1080/01904167.2012.721909.
[11] J. Pan, J. Zhang, C. Liu, S. Long, and L. Zhao, “Effects of Exogenous Calcium on Seed Germination and Physiological Traits of Alfalfa( Medicago sativa) Seedlings,” BMC Plant Biol., vol. 25, no. 313, pp. 1–14, 2025, doi: 10.1186/s12870-025-06334-y.
[12] Z. Yu et al., “Ultrasound-assisted Germination of Red Kidney Beans: Enhancements in Physicochemical and Nutritional Profiles,” Food Chem., vol. 454, p. 139829, Oct. 2024, doi: 10.1016/J.FOODCHEM.2024.139829.
[13] S. Elmiah et al., “Perlakuan Stres Pada Beras Kecambah Sebagai Strategi Peningkatan Senyawa Bioaktif Untuk Pengembangan Pangan Fungsional?: Kajian,” J. Sains dan Teknol. Pangan, vol. 10, no. 6, pp. 9086–9099, 2025.
[14] T. Oki et al., “Polymeric Procyanidins as Radical-scavenging Components in Red-hulled Rice,” J. Agric. Food Chem., vol. 50, no. 26, pp. 7524–7529, 2002, doi: 10.1021/jf025841z.
[15] Sumartini, Hasnelly, and Sarah, “Kajian Peningkatan Kualitas Beras Merah (Oryza sativa) Instan dengan Cara Fisik,” Pas. Food Technol. J., vol. 5, no. 1, p. 84, 2018, doi: 10.23969/pftj.v5i1.842.
[16] C. J. Bergman, Rice End-Use Quality Analysis, no. 1963. AACCI. Published by Elsevier Inc. in cooperation with AACC International, 2018. doi: 10.1016/B978-0-12-811508-4.00009-5.
[17] L. Lamberts, E. De Bie, G. E. Vandeputte, and W. S. Veraverbeke, “Food Chemistry Effect of Milling on Colour and Nutritional Properties of Rice,” vol. 100, pp. 1496–1503, 2007, doi: 10.1016/j.foodchem.2005.11.042.
[18] T. A. Wilson, R. J. Nicolosi, B. Woolfrey, and D. Kritchevsky, “Rice Bran Oil and Oryzanol Reduce Plasma Lipid and Lipoprotein Cholesterol Concentrations and Aortic Cholesterol Ester Accumulation to A Greater Extent Than Ferulic Acid in Hypercholesterolemic Hamsters B,” Nutr. Biochem., vol. 18, pp. 105–112, 2007, doi: 10.1016/j.jnutbio.2006.03.006.
[19] A. Matilla, M. Gallardo, and M. I. Puga-hermida, “Science Research?: Structural, Physiological and Molecular Aspects of Heterogeneity in Seeds: A Review,” Seed Sci. Res., vol. 15, no. 02, pp. 63–76, 2015, doi: 10.1079/SSR2005203.
[20] H. Nonogaki, G. W. Bassel, and J. D. Bewley, “Plant Science Germination — Still a Mystery,” Plant Sci., vol. 179, pp. 574–581, 2010, doi: 10.1016/j.plantsci.2010.02.010.
[21] P. Charoenthaikij, K. Jangchud, A. Jangchud, K. Piyachomkwan, P. Tungtrakul, and W. Prinyawiwatkhul, “Germination Conditions Affect Physicochemical Properties of Germinated Brown Rice Flour,” J. Food Sci., vol. 74, no. 9, pp. 658–665, 2009, doi: 10.1111/j.1750-3841.2009.01345.x.
[22] E. S. Ukpong, E. U. Onyeka, and B. S. Oladeji, “Bioactive compounds , nutrients and pasting properties of parboiled milled rice , brown rice and germinated brown rice of selected cultivars and the effects of germination durations,” vol. 2, no. February, 2023, doi: 10.1016/j.focha.2023.100234.
[23] T. Kaosa-ard and S. Songsermpong, “Influence of Germination Time on the GABA Content and Physical Properties of Germinated Brown Rice,” Asian J. Food Agro-Industry, vol. 5, no. 04, pp. 270–283, 2012.
[24] A. L. Asteraceae, G. Houle, L. Morel, C. E. Reynolds, and J. Siegel, “The Effect of Salinity on Different Developmental Stages of an Endemic Annual Plant, Aster laurentianus ( Asteraceae),” Am. J. Bot., vol. 88, no. 1, pp. 62–67, 2016.
[25] H. Munarko, S. Budijanto, and A. B. Sitanggang, “Pengaruh Waktu Perendaman Beras terhadap Profil Pasting dan Komponen Bioaktif Tepung Beras Pecah Kulit Berkecambah,” J. Pangan, vol. 30, no. 3, pp. 187–198, 2021.
[26] N. D. Yuliana and M. A. Akhbar, “Chemical and Physical Evaluation, Antioxidant and Digestibility Profiles of White and Pigmented Rice from Different Areas of Indonesia,” Brazilian J. Food Technol., vol. 23, pp. 1–13, 2020.
[27] K. Hosoda, H. Sasahara, K. Matsushita, and Y. Tamura, “Anthocyanin and Proanthocyanidin Contents, Antioxidant Activity, and in situ Degradability of Black and Red Rice Grains,” Asian-Austalasian J. Anim. Sci., vol. 31, no. 8, pp. 1213–1220, 2018.
[28] M. S. Sadimantara, F. N. Sadimantara, and N. Asyik, “Physicochemical And Antioxidant Properties Of Red Rice Varieties Of Wakawondu And Wangkariri From North Buton , Indonesia,” Int. J. Sci. Technol. Res., vol. 8, no. 08, 2019.
[29] D. P. R. Ascheri, J. A. Boeno, P. Z. Bassinello, and J. L. R. Ascheri, “Correlation Between Grain Nutritional Content and Pasting Properties of Pre-gelatinized Red Rice Flour,” vol. 59, no. 1, pp. 16–24, 2012.
[30] R. Raghuvanshi, A. Dutta, G. Tewari, and S. Suri, “Qualitative Characteristics of Red Rice and White Rice Procured from Local Market of Uttarakhand: A Comparative Study,” J. Rice Res., vol. 10, no. 1, pp. 49–53, 2017.
[31] A. Moongngarm and N. Saetung, “Comparison of Chemical Compositions and Bioactive Compounds of Germinated Rough Rice and Brown Rice,” Food Chem., vol. 122, no. 3, pp. 782–788, 2010, doi: 10.1016/j.foodchem.2010.03.053.
[32] S. Jiamyangyuen and B. Ooraikul, “The Physico-chemical, Eating and Sensorial Properties of Germinated Brown Rice,” J. Food, Agric. Environ., vol. 6, pp. 119–124, 2008.
[33] Q. Zhang, N. Liu, S. Wang, and L. Pan, “Effects of Germination and Aeration Treatment Following Segmented Moisture Conditioning on the ?-aminobutyric Acid Accumulation in Germinated Brown Rice,” Int. J. Agric. Biol. Eng., vol. 13, no. 5, pp. 234–240, 2020, doi: 10.25165/j.ijabe.20201305.5538.
[34] Y. Xing et al., “CaCl2 Priming Promotes Sorghum Seed Germination under Salt Stress by Activating Sugar Metabolism,” Plant Growth Regul., vol. 101, pp. 385–397, 2023.
[35] Y. Putri, E. Proamono, M. Kamal, and Y. C. Ginting, “Efek Lama Perendaman dan Konsentrasi CaCl2 Sebagai Osmopriming pada Kinerja Perkecambahan Benih Sorgum,” Agrotek Tropoka, vol. 11, no. 4, pp. 627–634, 2023.
[36] M. Sood, J. D. Bandral, N. Gupta, and R. K. Salgotra, “Effect of Germination on Functional and Nutritional Quality Characteristics of Brown Rice,” Int. J. Adv. Biochem. Res., vol. 8, no. 6, pp. 555–559, 2024.
[37] Castanares, “Priming with Calcium Chloride Improves Lettuce ( Lactuca sativa L .) Germination at High Temperatures,” Hortic. Argentina, vol. 38, no. 97, pp. 6–12, 2019.
[38] N. Hidayati, S. Bambang, J. Teknologi, H. Pertanian, F. Pertanian, and U. S. Maret, “Pengaruh Variasi Media Penyangraian Terhadap Kualitas Teh Beras Merah Sebagai Alternatif Minuman Fungsional,” J. Teknosains Pangan, vol. 2, no. 3, pp. 96–104, 2013.
[39] R. Fitriana, “Penetapan Kadar Antosianin Dan Formulasi Sediaan Blush On Compact Powder Ekstrak Beras Merah (Oryza Rufipogon Griff.),” J. Med. Nusant., vol. 1, no. 4, pp. 296–314, 2023.
[40] A. B. Sitanggang, M. Joshua, H. Munarko, F. Kusnandar, and S. Budijanto, “Increased ?-aminobutyric acid Content of Germinated Brown Rice Produced in Membrane Reactor,” Food Technol. Biotechnol., vol. 59, no. 3, pp. 295–305, 2021, doi: 10.17113/ftb.59.03.21.6846.
[41] S. Kraithong, S. Lee, and S. Rawdkuen, “Physicochemical and Functional Properties of Thai Organic Rice Flour,” J. Cereal Sci., 2017, doi: 10.1016/j.jcs.2017.10.015.
[42] H. Munarko, M. A. Kurnianto, J. Jariyah, A. Arwani, and R. M. Sari, “Physicochemical Properties, Sensory Profile, and Emotional Perception of Unpolished Organic Rice,” Polish J. Food Nutr. Sci., vol. 75, no. 2, pp. 144–158, 2025, doi: 10.31883/pjfns/203936.
[43] T. Likitwattanasade and P. Hongsprabhas, “Effect of Storage Proteins on Pasting Properties and Microstructure of Thai rice,” Food Res. Int., vol. 43, no. 5, pp. 1402–1409, 2010, doi: 10.1016/j.foodres.2010.04.011.
[44] B. H. Mohan, N. G. Malleshi, and T. Koseki, “LWT - Food Science and Technology Physico-chemical characteristics and non-starch polysaccharide contents of Indica and Japonica brown rice and their malts,” LWT - Food Sci. Technol., vol. 43, no. 5, pp. 784–791, 2010, doi: 10.1016/j.lwt.2010.01.002.
[45] N. Musita, “Kajian Kadar Aflatoksin dan Proksimat Tepung Jagung Nikstamalisasi pada Berbagai Lama Perendaman,” pp. 98–105.
[46] T. S. Zakiyah, S. Winarti, and R. Yulistiani, “Pengaruh Konsentrasi Ca(OH) 2 dan Suhu Pemasakan pada Proses Nikstamalisasi Tepung Jagung,” Teknol. Pangan Media Inf. dan Komun. Ilm. Teknol. Pertan., vol. 13, no. 36, pp. 175–186, 2022.
[47] K. O. Ã, K. Suzuki, Y. Yasui, and T. Kasumi, “Bio-functional Components in the Processed Pre-germinated Brown Rice by a Twin-screw Extruder,” J. Food Compos. Anal., vol. 18, pp. 303–316, 2005, doi: 10.1016/j.jfca.2004.10.003.
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