Application of Ultrasonic Waves as Pre-Treatment in the Extraction of Essential Oil from Basil Leaves (Ocimum Basilicum) Using Ultrasound-Microwave-Assisted Extraction (UMAE)
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This study investigated the effects of temperature and ultrasonic duration on the Ultrasound–Microwave Assisted Extraction (U-MAE) method for basil leaf essential oil. Key characteristics analysed included yield, density, refractive index, and colour, with results compared to commercial essential oil. The procedure involved drying basil leaves, applying ultrasonic pretreatment at 30- 70 °C for 5-60 minutes, and performing microwave-assisted extraction at 450 W for 30 minutes. Findings indicated that both temperature and ultrasonic duration significantly influenced essential oil characteristics. Optimal conditions were achieved at 50 °C and 30 minutes, resulting in a yield of 2.5551%, a density of 0.9542 g/ml, a refractive index of 1.4960, and a pale yellow color consistent with commercial standards.
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[1] G. ?ukowska and Z. Durczy?ska, “Properties and Applications of Essential Oils: A Review,” J. Ecol. Eng., vol. 25, no. 2, pp. 333–340, 2024, doi: 10.12911/22998993/177404.
[2] M. R. Yan, C. H. Wang, N. J. C. Flores, and Y. Y. Su, “Targeting open market with strategic business innovations: A case study of growth dynamics in essential oil and aromatherapy industry,” J. Open Innov. Technol. Mark. Complex., vol. 5, no. 1, p. 7, 2019, doi: 10.3390/joitmc5010007.
[3] D. K. Y. Putri, S. A. Putri, B. A. Nugraha, B. A. Fachri, and B. Palupi, “Extraction of Basil Leaves Essential Oil using Microwave Assisted Hydrodistillation Method: Physical Characterization and Antibacterial Activity,” CHEESA Chem. Eng. Res. Artic., vol. 6, no. 1, p. 1, 2023, doi: 10.25273/cheesa.v6i1.14639.1-12.
[4] P. Fajar, K. Lestari, A. Ferhat, M. P. Bimantio, and U. Mahasaraswati, “COMPETITIVENESS ANALYSIS OF INDONESIAN ESSENTIAL OIL AS AN EXPORT,” vol. 7, no. March, pp. 144–154, 2023.
[5] T. Tanrisannah, A. Febryanti, and S. Chadijah, “Ekstraksi Minyak Daun Kemangi (Occmum basilicum L.) Menggunakan Metode Microwave Assisted Hydrodistillation,” J. Keteknikan Pertan. Trop. dan Biosist., vol. 11, no. 3, pp. 296–304, 2023, doi: 10.21776/ub.jkptb.2023.011.03.07.
[6] M. Silalahi, “MINYAK ESSENSIAL PADA KEMANGI (Ocimum basilicum L.),” J. Pro-Life, vol. 5, no. 2, pp. 557–561, 2018.
[7] E. Da Silva Moura, L. R. D. A. Faroni, F. F. Heleno, A. A. Z. Rodrigues, L. H. F. Prates, and M. E. L. R. De Queiroz, “Optimal extraction of Ocimum basilicum essential oil by association of ultrasound and hydrodistillation and its potential as a biopesticide against a major stored grains pest,” Molecules, vol. 25, no. 12, pp. 1–16, 2020, doi: 10.3390/molecules25122781.
[8] N. S. Azizah et al., “Sweet Basil (Ocimum basilicum L.)?A Review of Its Botany, Phytochemistry, Pharmacological Activities, and Biotechnological Development,” Plants, vol. 12, no. 24, 2023, doi: 10.3390/plants12244148.
[9] W. Max, S. Dianini, H. Kringel, E. Júlia, and D. De Souza, “Basil Essential Oil?: Methods of Extraction , Chemical Composition , Biological Activities , and Food Applications,” Food Bioprocess Technol., no. 0123456789, 2021, doi: 10.1007/s11947-021-02690-3.
[10] N. ?or?evi? et al., “The effect of distillation methods on the yield, composition and biological activity of basil (Ocimum basilicum L.) essential oil,” Adv. Technol., vol. 11, no. 2, pp. 16–25, 2022, doi: 10.5937/savteh2202016d.
[11] T. P. Vo et al., “Optimization of Ultrasonic-Assisted Extraction of Phenolics and Terpenoids from Sweet Basil Leaves Using Natural Deep Eutectic Solvents,” vol. 2024, 2024, doi: 10.1155/2024/5199884.
[12] J. Senrayan and S. Venkatachalam, “Ultrasonic acoustic-cavitation as a novel and emerging energy e ffi cient technique for oil extraction from kapok seeds,” Innov. Food Sci. Emerg. Technol., vol. 62, no. November 2019, p. 102347, 2020, doi: 10.1016/j.ifset.2020.102347.
[13] R. C. N. T. Lee, F. Siow, T. T. Yee, and Y. Lee, “A review on application of ultrasound and ultrasound assisted technology for seed oil extraction,” J. Food Sci. Technol., vol. 60, no. 4, pp. 1222–1236, 2023, doi: 10.1007/s13197-022-05359-7.
[14] B. Gotama, D. F. Rahman, and A. F. Anjarwadi, “Intensifikasi Proses Penyulingan Minyak Atsiri dari Daun Jeruk Purut dengan Metode Ultrasound following Microwave Assisted Extraction (US-MAE),” Indones. J. Essent. Oil, vol. 2, no. 1, pp. 29–37, 2017.
[15] Y. Ayu, T. Wahyuni, G. Ayu, K. Diah, and I. N. K. Putra, “Pengaruh Jenis Pelarut pada Metode Microwave Assisted Extraction ( MAE ) terhadap Karakteristik Ekstrak Daun Singkong ( Manihot utilissima Pohl .) The Effect of Type of Solvent Using Microwave Assisted Extraction ( MAE ) Method on The Characteristics of Cassave Leaf Extract ( Manihot utilissima Pohl .),” vol. 10, no. 4, pp. 566–578, 2021.
[16] A. P. Wicaksono, F. A. Afandi, C. Pujiastuti, K. Erliyanti, and R. Panjaitan, “Rekayasa Bahan Alam dan Energi Berkelanjutan Ultrasound Microwave Assisted Extraction Citronella Leaves Using Ionic Solvent on,” vol. 08, no. 1, pp. 42–49, 2024.
[17] J. Adiandasari, “PENGARUH SUHU DAN WAKTU TERHADAP PROSES PENYULINGAN MINYAK SEREH WANGI,” vol. 1, no. Agustus, pp. 22–28, 2021.
[18] R. Qorriaina, L. C. Hawa, and R. Yulianingsih, “Jurnal Bioproses Komoditas Tropis Aplikasi Pra-Perlakuan Microwave Assisted Extraction ( MAE ) Pada Ekstrak Daun Kemangi ( Ocimum sanctum ) Menggunakan Rotary Evaporator ( Studi Pada Variasi Suhu dan Waktu Ekstraksi ) The Application of Microwave Assisted,” vol. 3, no. 1, pp. 32–38, 2015.
[19] A. P. Rosa and B. Albero, “Trends in Analytical Chemistry Ultrasound-assisted extraction methods for the determination of organic contaminants in solid and liquid samples,” vol. 166, no. April, 2023.
[20] N. K. Erliyanti et al., “PENGARUH DAYA MICROWAVE TERHADAP YIELD PADA EKSTRAKSI MINYAK ATSIRI DARI BUNGA KAMBOJA ( PLUMERIA ALBA ) MENGGUNAKAN METODE MICROWAVE HYDRODISTILLATION,” vol. 8, no. 3, pp. 175–178, 2017.
[21] M. Firdaus et al., “Peningkatan Rendemen Minyak Atsiri Melalui Maserasi Awal dengan Metode Ultrasonikasi di Rumah Atsiri Indonesia,” PRIMA J. Community Empower. Serv., vol. 9, no. 1, p. 50, 2025, doi: 10.20961/prima.v9i1.86385.
[22] B. Susilo, S. H. Sumarlan, Y. Wibisono, and N. Puspitasari, “Pengaruh Pretreatment dan Lama Waktu Ekstraksi terhadap Karakteristik Ekstrak Kulit Jeruk Purut ( Citrus hystrix D . C ) Menggunakan Ultrasonic Assisted Extraction ( UAE ) Effect of Pretreatment and Extraction Duration to the Characteristics of Kaffir Lim,” J. Keteknikan Pertan. Trop. dan Biosist., vol. 4, no. 3, pp. 230–241, 2018, [Online]. Available: https://jkptb.ub.ac.id/index.php/jkptb/article/view/383
[23] B. Gotama and A. Sasongko, “Intensifikasi Proses pada Penyulingan Minyak Serai Wangi dengan Bantuan Gelombang Ultrasonik ( Ultrasonic following Steam-Hydro Distillation ),” vol. 6, no. 1, pp. 3–10, 2020.
[24] M. G. Teke, L. De Vos, I. Smith, T. Kleyn, and Z. Mapholi, “Development of an ultrasound ? assisted pre ? treatment strategy for the extraction of d ? Limonene toward the production of bioethanol from citrus peel waste ( CPW ),” Bioprocess Biosyst. Eng., vol. 46, no. 11, pp. 1627–1637, 2023, doi: 10.1007/s00449-023-02924-y.
[25] N. K. Erliyanti, A. D. Priyanto, C. Pujiastuti, and J. Timur Program Studi Teknik Kimia, “KARAKTERISTIK DENSITAS DAN INDEKS BIAS MINYAK ATSIRI DAUN JAMBU KRISTAL (Psidium Guajava) MENGGUNAKAN METODE MICROWAVE HYDRODISTILLATION DENGAN VARIABEL DAYA DAN RASIO BAHAN?: PELARUT,” J. Rekayasa Mesin, vol. 11, no. 2, pp. 247–255, 2020.
[26] E. S. A. Fawaid, L I Sari, C Pujiastuti, N K Erliyanti, A D Priyanto, and E A Saputro, “Aplikasi Portable Brix Meter untuk Perhitungan Indeks Bias Minyak Atsiri Daun Jambu Kristal,” Biomed. Mech. Eng. J., vol. 1, no. 1, p. 4, 2021, doi: 10.33005/biomej.v1i1.24.
[27] I. Syauqiah, A. Mirwan, A. Sulaiman, and N. Nurandini, “Info – Teknik Analisis Pengaruh Lama Penyulingan Dan Komposisi Bahan Baku Terhadap Rendemen Dan,” vol. 9, no. 1, pp. 21–30, 2018.
[28] L. Mugao, “Factors influencing yield , chemical composition and efficacy of essential oils,” pp. 169–178, 2024.
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